建立小鼠肾移植模型视频大小113M

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建立小鼠肾移植模型视频大小113MMurine Renal Transplantation ProcedureAnesthesia is maintained by Isoflurane (2-3%). 10-0 suture for anastomoses.
Make an end-to-side anastomosis between the donor aortic cuff and the anterior wall of the recipient’s aortaotomy, using 2 ~ 3 continuous 10-0 nylon sutures in the outside of the aorta.Turn the donor kidney graft over to the left flank of the recipient. repeat the previous procedure between the donor aortic cuff and the posterior wall of the recipient’s aortaotomy.
Perform an end-to-side anastomosis between the donor renal vein and the posterior wall of the recipient’s IVC, using 4 to 5 continuous 10-0 nylon sutures in the inside of the IVC. and then the anterior wall of IVC and donor’s renal vein are closed with 4 ~ 5 continuous sutures outside the IVC.
Urinary reconstruction via a bladder-to-bladder anastomosis.


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